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A reusable tamper-evident envelope includes a first substrate, a second substrate superimposed on the first substrate, at least one auxiliary substrate superimposed on and joined along one edge to the second substrate, and at least one seal along all but one edge joining the first substrate to the second substrate. Each auxiliary substrate is spaced a user-defined distance from the top edge of the first substrate. A perforation line, extending from the left side to the right side of both the first substrate and second substrates facilitate removal of a portion of the respective substrates. After an item is placed into the envelope, the user-activated seal along the remaining edge joins the first substrate to the second substrate. Subsequent uses are possible by removing portions of the first substrate and second substrate along the perforation lines and activating the auxiliary user-activated seal along the auxiliary substrate joining the first substrate to the second substrate.